Dore M P, Osato M S, Malaty H M, Graham D Y
Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas 77030-4298, USA.
Helicobacter. 2000 Sep;5(3):165-8. doi: 10.1046/j.1523-5378.2000.00026.x.
Helicobacter pylori is difficult to culture from stool. Multiple efforts from multiple laboratories have been unsuccessful, and the optimal conditions to recover H. pylori from stool are still not known. Recovery of H. pylori from feces of infected individuals is important for the performance of molecular epidemiological investigations, especially in children, where their symptoms do not warrant endoscopy to recover the organism.
Fresh fecal specimens (noncathartic) were obtained from 19 known H. pylori-infected patients and were processed to recover the organism. Fresh fecal specimens (noncathartic) were also obtained from three known H. pylori-negative individuals (controls) to determine whether H. pylori could be isolated from stools seeded with known concentrations of the organism. Treatment of the fecal suspensions with cholestyramine, a basic anion exchange resin that binds bile acids, was used in an attempt to enhance recovery of H. pylori by sequestering bile acids that are inhibitory to H. pylori growth. H. pylori was identified based on colony morphology, cell morphology, Gram's stain, biochemical reactions, and polymerase chain reaction for two H. pylori genes.
Among 19 patients, H. pylori was cultured at least once from 3 and three times from 2 (5 of 19). Feces that were seeded with H. pylori and obtained from three H. pylori-negative volunteer controls yielded positive recovery in all instances.
We have confirmed that it is possible to culture H. pylori from human stool, but the procedure for optimal recovery has still not been defined.
从粪便中培养幽门螺杆菌很困难。多个实验室进行的多次尝试均未成功，从粪便中分离出幽门螺杆菌的最佳条件仍不清楚。从受感染个体的粪便中分离出幽门螺杆菌对于开展分子流行病学调查很重要，尤其是在儿童中，因为他们的症状不需要通过内镜检查来获取该病原体。
从19名已知感染幽门螺杆菌的患者中获取新鲜粪便标本（未使用泻药），并对其进行处理以分离出该病原体。还从3名已知幽门螺杆菌阴性个体（对照）中获取新鲜粪便标本（未使用泻药），以确定是否能从接种已知浓度该病原体的粪便中分离出幽门螺杆菌。使用消胆胺（一种结合胆汁酸的碱性阴离子交换树脂）处理粪便悬液，试图通过螯合抑制幽门螺杆菌生长的胆汁酸来提高幽门螺杆菌的回收率。根据菌落形态、细胞形态、革兰氏染色、生化反应以及针对两个幽门螺杆菌基因的聚合酶链反应来鉴定幽门螺杆菌。
在19名患者中，有3名患者的粪便至少培养出一次幽门螺杆菌，2名患者的粪便培养出三次（19名患者中的5名）。从三名幽门螺杆菌阴性志愿者对照中获取的接种了幽门螺杆菌的粪便在所有情况下均获得了阳性培养结果。
我们已证实可以从人类粪便中培养出幽门螺杆菌，但最佳回收率的程序仍未确定。
