Kato C, Sato T, Smorawinska M, Horikoshi K
Deep Star Group, Japan Marine Science and Technology Center, Yokosuka.
FEMS Microbiol Lett. 1994 Sep 15;122(1-2):91-6. doi: 10.1111/j.1574-6968.1994.tb07149.x.
Recombinant plasmids with the chloramphenicol acetyltransferase (CAT) structural gene behind several kinds of promoters were tested for expression in Escherichia coli during growth at atmospheric pressure (0.1 MPa) and at high pressure (30 MPa). Expression of the CAT gene from the lac promoter was remarkably activated (approx. 78-fold) by high pressure in the absence of the inducer isopropyl-beta-D-thiogalactopyranoside (IPTG). The stimulation of the CAT activity by the lac promoter at high pressure did not simply result from an increased plasmid copy number, because the CAT activities from the other promoters and beta-lactamase activities were unaffected at high pressure.
对几种启动子下游带有氯霉素乙酰转移酶（CAT）结构基因的重组质粒，在大肠杆菌于常压（0.1MPa）和高压（30MPa）下生长期间进行了表达测试。在没有诱导剂异丙基-β-D-硫代半乳糖苷（IPTG）的情况下，高压显著激活了来自乳糖启动子的CAT基因表达（约78倍）。高压下乳糖启动子对CAT活性的刺激并非简单地源于质粒拷贝数增加，因为来自其他启动子的CAT活性和β-内酰胺酶活性在高压下不受影响。
