Kato Naoto, Sato Takako, Kato Chiaki, Yajima Masao, Sugiyama Junji, Kanda Takahisa, Mizuno Masahiro, Nozaki Kouichi, Yamanaka Shigeru, Amano Yoshihiko
Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University, 4-17-1 Wakasato, Nagano, 380-8553, Japan.
Extremophiles. 2007 Sep;11(5):693-8. doi: 10.1007/s00792-007-0085-y. Epub 2007 Jul 21.
The effect of pressure on viability and the synthesis of bacterial cellulose (BC) by Gluconacetobacter xylinus ATCC53582 were investigated. G. xylinus was statically cultivated in a pressurized vessel under 0.1, 30, 60, and 100 MPa at 25 degrees C for 6 days. G. xylinus cells remained viable and retained cellulose producing ability under all the conditions tested, though the production of cellulose decreased with increasing the pressure. The BCs produced at each pressure condition were analyzed by field emission scanning electron microscopy (FE-SEM) and Fourier Transform Infrared (FT-IR). FE-SEM revealed that the widths of BC fibers produced under high pressure decreased as compared with those produced under the atmospheric pressure. By FT-IR, all the BCs were found to be of Cellulose type I, as the same as typical native cellulose. Our findings evidently showed that G. xylinus possessed a piezotolerant (barotolerant) feature adapting to 100 MPa without losing its BC producing ability. This was the first attempt in synthesizing BC with G. xylinus under elevated pressure of 100 MPa, which corresponded to the deep sea at 10,000 m.
研究了压力对木醋杆菌ATCC53582的活力以及细菌纤维素（BC）合成的影响。木醋杆菌在25℃下于0.1、30、60和100MPa的压力下在加压容器中静置培养6天。在所有测试条件下，木醋杆菌细胞均保持活力并保留纤维素生产能力，尽管纤维素的产量随着压力的增加而降低。通过场发射扫描电子显微镜（FE-SEM）和傅里叶变换红外光谱（FT-IR）对在每个压力条件下产生的细菌纤维素进行了分析。FE-SEM显示，与在大气压下产生的细菌纤维素纤维宽度相比，高压下产生的细菌纤维素纤维宽度减小。通过FT-IR发现，所有细菌纤维素均为I型纤维素，与典型的天然纤维素相同。我们的研究结果清楚地表明，木醋杆菌具有耐压（耐气压）特性，能够适应100MPa的压力而不丧失其产生细菌纤维素的能力。这是首次在100MPa的高压下用木醋杆菌合成细菌纤维素，该压力相当于10000米深海的压力。
