Saianova O V, Mekhedov S L, Burkitbaev E M, Shchikova N I, Anan'ev E V
Genetika. 1994 Jun;30(6):749-55.
Three nucleotide sequences from the barley genome, containing the C-hordein genes lambda CH1, lambda CH3, and lambda CH5, were cloned. They were shown to have different physical maps, and the structural organization of homologous sequences was found to be highly conservative. The surrounding sequences of C-hordein genes contain insertions specific in length and copy number. A Hind III fragment of about 3000 bp in size of the lambda CH5 clone contains a highly repeated nucleotide sequence. The lambda CH3 clone contains two "head-to-head" repeated C-hordein genes. This is the first evidence that hordein genes may be located in the immediate vicinity of each other in a 17,800-bp region. A 919-bp nucleotide sequence of one of the C-hordein genes from this clone, pCHOR3, was determined. An open reading frame of 363 bp codes for a potential polypeptide consisting of 121 amino acids. The main part of the mature polypeptide consists of repeated octapeptide Pro-Gln-Gln-Pro-Phe-Pro-Gln-Gln motifs. The coding region has no introns. The 5' untranslated region contains regulatory loci specific for prolamin genes: the -300 element and the CAAT, AGGA, and TATA boxes. Significant differences in the lengths of the coding regions of cloned genes were observed: 363 and 1000 bp in the lambda CH3 clone, 500 bp in the lambda CH1 clone, and 600 bp in the lambda CH5 clone, which may be a molecular cause of C-hordein size polymorphism.
从大麦基因组中克隆出了三个包含C-醇溶蛋白基因λCH1、λCH3和λCH5的核苷酸序列。结果表明它们具有不同的物理图谱，并且发现同源序列的结构组织高度保守。C-醇溶蛋白基因的周边序列包含长度和拷贝数特异的插入片段。λCH5克隆中一个大小约为3000 bp的Hind III片段包含一个高度重复的核苷酸序列。λCH3克隆包含两个“头对头”重复的C-醇溶蛋白基因。这是醇溶蛋白基因可能在一个17,800 bp区域内彼此紧邻定位的首个证据。测定了来自该克隆pCHOR3的一个C-醇溶蛋白基因的919 bp核苷酸序列。一个363 bp的开放阅读框编码一个由121个氨基酸组成的潜在多肽。成熟多肽的主要部分由重复的八肽Pro-Gln-Gln-Pro-Phe-Pro-Gln-Gln基序组成。编码区没有内含子。5'非翻译区包含醇溶蛋白基因特异的调控位点：-300元件以及CAAT、AGGA和TATA框。观察到克隆基因编码区长度存在显著差异：λCH3克隆中为363和1000 bp，λCH1克隆中为500 bp，λCH5克隆中为600 bp，这可能是C-醇溶蛋白大小多态性的分子原因。
