Kim M H, Aimar C, Best-Belpomme M, Maisonhaute C
Groupe de Génétique Cellulaire et Moléculaire, URA-CNRS 1135, Université Pierre et Marie Curie-7, Paris, France.
Genetica. 1994;92(2):107-14. doi: 10.1007/BF00163759.
The entire 1731 retrotransposon of Drosophila melanogaster, tagged with the E. coli lac Z gene inserted in its gag sequence, was injected into oocytes and fertilized eggs of the urodele amphibian Pleurodeles waltl. Expression of the reporter gene indicated that the 1731 promoter (its 5'LTR) is active in the embryos and not in the oocytes. It appeared that this element is regulated as amphibian genes are at the beginning of the development, i.e. that expression was detected after the mid blastula stage and maintained up to four or five days after injection. Another construction associating the modified 1731 promoter with the CAT gene is also expressed in Pleurodeles embryos during the same period of development. This indicated that the 1731 promoter issued from a Drosophila species is activated as promoting sequences of amphibian zygotic genes are, suggesting that in the case of horizontal transfer, 1731 can be expressed into vertebrate organisms.
将插入了大肠杆菌lac Z基因且标记于其gag序列的黑腹果蝇完整1731反转录转座子，注射到有尾两栖动物肋突螈的卵母细胞和受精卵中。报告基因的表达表明，1731启动子（其5'长末端重复序列）在胚胎中具有活性，而在卵母细胞中无活性。看起来该元件的调控方式与两栖动物基因在发育初期的调控方式相同，即表达在囊胚中期之后被检测到，并在注射后持续四到五天。另一个将修饰后的1731启动子与CAT基因相连的构建体，在肋突螈胚胎发育的同一时期也有表达。这表明源自果蝇物种的1731启动子，如同两栖动物合子基因的启动序列一样被激活，这意味着在水平转移的情况下，1731能够在脊椎动物体内表达。
