Cadoret J P, Gendreau S, Delecheneau J M, Rousseau C, Mialhe E
IFREMER-CNRS-Université de Montpellier II, France.
Mol Mar Biol Biotechnol. 1997 Mar;6(1):72-7.
New strategies for embryonic manipulation have been developed in recent years through plant and animal research. However, research on marine invertebrate embryos has suffered from a lack of basic tools, such as microinjection. Here we present a technique developed for microinjecting eggs and embryos of the oyster Crassostrea gigas and the mussel Mytilus edulis. In experimental trials, approximately 40% of microinjected embryos survived. This technique was used to microinject beta-galactosidase, for which specific detection techniques were developed. A reporter construct (CMV-beta) based on a promoter of cytomegalovirus linked to the beta-galactosidase-encoding gene was then microinjected, and the expression level of this construct was monitored. The suitability of this technique is discussed in terms of its application to the manipulation of bivalve mollusks in pathology and genetics.
近年来，通过植物和动物研究，已经开发出了用于胚胎操作的新策略。然而，对海洋无脊椎动物胚胎的研究一直缺乏基本工具，如显微注射技术。在此，我们展示了一种为牡蛎巨蛎和贻贝紫贻贝的卵和胚胎显微注射而开发的技术。在实验试验中，约40%的显微注射胚胎存活。该技术被用于显微注射β-半乳糖苷酶，并开发了针对该酶的特异性检测技术。然后，将基于巨细胞病毒启动子与β-半乳糖苷酶编码基因相连的报告基因构建体（CMV-β）进行显微注射，并监测该构建体的表达水平。本文从该技术在双壳贝类病理学和遗传学操作中的应用角度，讨论了其适用性。
