Ma Z W, Bergemann A D, Johnson E M
Department of Pathology, Mount Sinai School of Medicine, New York, NY 10029.
Gene. 1994 Nov 18;149(2):311-4. doi: 10.1016/0378-1119(94)90167-8.
Pur alpha is a sequence-specific single-stranded DNA-binding protein with affinity for an element present in several eukaryotic origins of DNA replication (ori) and gene regulatory regions. We report here the cDNA sequence for mouse pur alpha and an extraordinary degree of conservation between human and mouse Pur alpha (hPurA and mPurA, respectively). There are only two single-amino-acid (aa) changes between hPurA (322 aa) and mPurA (321 aa). One PurA region of 22 aa, termed the 'psycho' motif, possesses significant homology to a counterpart in the SV40 large T-antigen, to several other transforming proteins of DNA tumor viruses, and to certain cellular proteins in yeast and human cells that may also be involved in the initiation of DNA replication.
Purα是一种序列特异性单链DNA结合蛋白，对几种真核生物DNA复制起点（ori）和基因调控区域中存在的一种元件具有亲和力。我们在此报告小鼠Purα的cDNA序列，以及人和小鼠Purα（分别为hPurA和mPurA）之间的高度保守性。hPurA（322个氨基酸）和mPurA（321个氨基酸）之间只有两个单氨基酸（aa）变化。一个由22个氨基酸组成的PurA区域，称为“psycho”基序，与SV40大T抗原中的对应区域、DNA肿瘤病毒的其他几种转化蛋白以及酵母和人类细胞中某些可能也参与DNA复制起始的细胞蛋白具有显著同源性。
