大肠杆菌O111:H-中插入序列IS1203的特性分析
Characterization of IS1203, an insertion sequence in Escherichia coli O111:H-.
作者信息
Paton A W, Paton J C
机构信息
Department of Microbiology, Women's and Children's Hospital, North Adelaide, Australia.
出版信息
Gene. 1994 Dec 2;150(1):67-70. doi: 10.1016/0378-1119(94)90859-1.
The complete nucleotide (nt) sequence of IS1203 from Escherichia coli O111:H- strain PH has been determined. IS1203 is 1312-nt long, with imperfect 26-bp terminal inverted repeats. The two major ORFs in IS1203 encode polypeptides of 12.7 and 33.7 kDa, the latter being the putative transposase. IS1203 is closely related to IS629 of Shigella sonnei and IS3411 of E. coli. At least twelve copies of IS1203 were found in the genome of E. coli O111:H- strain PH.
已测定来自大肠杆菌O111:H-菌株PH的IS1203的完整核苷酸（nt）序列。IS1203长1312 nt，具有不完美的26 bp末端反向重复序列。IS1203中的两个主要开放阅读框（ORF）编码12.7 kDa和33.7 kDa的多肽，后者是推定的转座酶。IS1203与宋内志贺菌的IS629和大肠杆菌的IS3411密切相关。在大肠杆菌O111:H-菌株PH的基因组中发现了至少12个IS1203拷贝。
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