Prère M F, Chandler M, Fayet O
Centre de Biochimie et Génétique Cellulaires du Centre National de la Recherche Scientifique, Toulouse, France.
J Bacteriol. 1990 Jul;172(7):4090-9. doi: 10.1128/jb.172.7.4090-4099.1990.
Twenty-nine clear-plaque mutants of bacteriophage lambda were isolated from a Shigella dysenteriae lysogen. Three were associated with insertions in the cI gene: two were due to insertion of IS600, and the third resulted from insertion of a new element, IS911. IS911 is 1,250 base pairs (bp) long, carries 27-bp imperfect terminal inverted repeats, and generates 3-bp duplications of the target DNA on insertion. It was found in various copy numbers in all four species of Shigella tested and in Escherichia coli K-12 but not in E. coli W. Analysis of IS911-mediated cointegrate molecules indicated that the majority were generated without duplication of IS911. They appeared to result from direct insertion via one end of the element and the neighboring region of DNA, which resembles a terminal inverted repeat of IS911. Nucleotide sequence analysis revealed that IS911 carries two consecutive open reading frames which code for potential proteins showing similarities to those of the IS3 group of elements.
从一株痢疾志贺氏菌溶原菌中分离出了29个噬菌体λ的清晰噬菌斑突变体。其中三个与cI基因中的插入有关：两个是由于IS600的插入，第三个是由一个新元件IS911插入所致。IS911长1250个碱基对（bp），带有27 bp的不完全末端反向重复序列，插入时会在靶DNA上产生3 bp的重复序列。在所有检测的四种志贺氏菌以及大肠杆菌K-12中均发现了不同拷贝数的IS911，但在大肠杆菌W中未发现。对IS911介导的共整合分子的分析表明，大多数共整合分子的产生并未伴随IS911的重复。它们似乎是通过元件的一端与相邻的DNA区域直接插入而产生的，这类似于IS911的末端反向重复序列。核苷酸序列分析表明，IS911带有两个连续的开放阅读框，它们编码的潜在蛋白质与IS3元件组的蛋白质具有相似性。
