Kannan K, Jain S K
Department of Pediatrics, Louisiana State University School of Medicine, Shreveport.
Horm Metab Res. 1994 Jul;26(7):322-5. doi: 10.1055/s-2007-1001695.
Nephropathy is common among the diabetic population. However, the molecular mechanisms by which hyperglycemia can cause alteration in kidney structure and function is not known. In this study, we examined the effect of high glucose levels on cellular growth and membrane lipid peroxidation in Vero cells, an African green monkey kidney cell line. Cell growth was assessed by a tetrazolium salt reduction test (MTT); and lipid peroxidation was measured by thiobarbituric acid reactivity. Our results show that elevated levels of glucose (25, 50 and 100 mM) can cause up to 50% reduction in cell growth in cultured Vero cells as compared with controls (8 mM). Also, we observed a significant increase in the amount of oxidative damage in Vero cells cultured with elevated glucose concentrations. This study demonstrates that hyperglycemia can cause increased lipid peroxidation and affect the cellular proliferation in a monkey kidney cell line.
肾病在糖尿病患者群体中很常见。然而，高血糖导致肾脏结构和功能改变的分子机制尚不清楚。在本研究中，我们检测了高糖水平对非洲绿猴肾细胞系Vero细胞的细胞生长和膜脂质过氧化的影响。通过四唑盐还原试验（MTT）评估细胞生长；通过硫代巴比妥酸反应性测定脂质过氧化。我们的结果表明，与对照组（8 mM）相比，升高的葡萄糖水平（25、50和100 mM）可导致培养的Vero细胞的细胞生长减少高达50%。此外，我们观察到在高葡萄糖浓度培养的Vero细胞中氧化损伤量显著增加。本研究表明，高血糖可导致脂质过氧化增加并影响猴肾细胞系中的细胞增殖。
