Affara N A, Bentley E, Davey P, Pelmear A, Jones M H
Department of Pathology, University of Cambridge, United Kingdom.
Genomics. 1994 Jul 1;22(1):205-10. doi: 10.1006/geno.1994.1363.
To facilitate the characterization of genetic expression in human adult testis, expressed sequence tag analysis of cDNAs from this tissue has been undertaken. Over 180 kb of DNA sequence has been determined and used to search the GenBank database. The results from the first 359 cDNA clones analyzed indicate that the sequences could be sorted into several categories with a high proportion being novel. Twenty-five clones (7%) showed 100% identity with human genes, 11 (3%) with prokaryotic sequences, 21 (5%) with between 60 and 95% similarity to human genes, 27 (8%) with between 60 and 95% similarity to genes from other species, and 33 (9%) with matches to human repeat sequences. Two hundred forty-two (67%) showed no significant matches and thus are likely to represent novel transcripts. In comparison to similar studies on human brain tissue and a hepatoma cell line, the findings indicate that the matches in the testis transcript population appear to be identifying a different spectrum of gene sequences.
为便于对人类成年睾丸中的基因表达进行特征分析，已对该组织的cDNA进行了表达序列标签分析。已测定了超过180 kb的DNA序列，并用于搜索GenBank数据库。对最初分析的359个cDNA克隆的结果表明，这些序列可分为几类，其中很大一部分是新的。25个克隆（7%）与人类基因具有100%的同一性，11个（3%）与原核序列相同，21个（5%）与人类基因有60%至95%的相似性，27个（8%）与其他物种的基因有60%至95%的相似性，33个（9%）与人类重复序列匹配。242个（67%）没有明显匹配，因此可能代表新的转录本。与对人类脑组织和肝癌细胞系的类似研究相比，这些发现表明，睾丸转录本群体中的匹配似乎在识别不同的基因序列谱。
