Jutila D B, Kurk S, Jutila M A
Montana State University, Bozeman 59717.
Immunol Lett. 1994 Jun;41(1):49-57. doi: 10.1016/0165-2478(94)90056-6.
All leukocytes, except some subsets of lymphocytes, express Ly-6C. However, virtually all of the regulation studies of this antigen have been done on lymphocytes only. Recently we showed that monocytes and neutrophils express 10-100 times the level of Ly-6C expressed by lymphoid cells. We compared Ly-6C expression on neutrophils and monocytes following short-term cell activation induced by C5a or phorbol esters or treatment with phosphatidylinositol-specific phospholipase-C (PI-PLC). Activation of the neutrophil led to a rapid ( < 30 min) increase in surface expression of Ly-6C, but similar treatment had little effect on or slightly diminished the expression of the monocyte molecule. Prolonged treatment of the monocyte with IFN-gamma (24 h) led to increased Ly-6C expression. Low doses of TNF-alpha inhibited the up-regulation of Ly-6C induced by IFN-gamma. Similar to what has been described for the lymphocyte, the monocyte form of Ly-6C was hydrolyzed by PI-PLC treatment within 30 min. In contrast, the neutrophil molecule was unaffected by PI-PLC for up to 2 h. SDS-PAGE Western blot analysis demonstrated that the neutrophil and monocyte molecules were in the same molecular weight range (14-18 kDa Mr). Our results clearly show differences in the regulation of Ly-6C expression on monocytes versus neutrophils. Thus, it is likely that the potential function of Ly-6C will be unique for each cell.
除某些淋巴细胞亚群外，所有白细胞均表达Ly-6C。然而，实际上对该抗原的所有调控研究仅在淋巴细胞上进行。最近我们发现，单核细胞和中性粒细胞表达的Ly-6C水平是淋巴细胞的10至100倍。我们比较了在C5a或佛波酯诱导的短期细胞活化后，或用磷脂酰肌醇特异性磷脂酶-C（PI-PLC）处理后，中性粒细胞和单核细胞上Ly-6C的表达。中性粒细胞的活化导致Ly-6C表面表达迅速（<30分钟）增加，但类似处理对单核细胞分子的表达几乎没有影响或略有降低。用IFN-γ对单核细胞进行长时间（24小时）处理会导致Ly-6C表达增加。低剂量的TNF-α抑制IFN-γ诱导的Ly-6C上调。与淋巴细胞的情况类似，单核细胞形式的Ly-6C在PI-PLC处理后30分钟内被水解。相比之下，中性粒细胞分子在长达2小时内不受PI-PLC影响。SDS-PAGE Western印迹分析表明，中性粒细胞和单核细胞分子的分子量范围相同（Mr为14 - 18 kDa）。我们的结果清楚地显示了单核细胞与中性粒细胞在Ly-6C表达调控上的差异。因此，Ly-6C的潜在功能在每个细胞中可能都是独特的。
