Ly-6.A2在小鼠角质形成细胞上的组成性表达以及在TCRγδ⁺树突状表皮T细胞上的诱导性表达。

Constitutive expression of Ly-6.A2 on murine keratinocytes and inducible expression on TCR gamma delta+ dendritic epidermal T cells.

作者信息

HogenEsch H, de Geus B, Tielen F, Rozing J

机构信息

Department of Pathology and Immunology, TNO-Institute for Aging and Vascular Research, Leiden, The Netherlands.

出版信息

J Dermatol Sci. 1993 Apr;5(2):114-21. doi: 10.1016/0923-1811(93)90079-5.

DOI:10.1016/0923-1811(93)90079-5

PMID:8102878

Abstract

We investigated the expression of Ly-6.A2 on isolated murine epidermal cells by flow cytometry. Ly-6.A2 was expressed on 61% of keratinocytes and 6% of dendritic epidermal T cells of C57BL mice. Phosphatidylinositol-specific phospholipase C removed Ly-6.A2, indicating that the antigen is anchored to the keratinocyte membrane via a glycosyl-phosphatidylinositol anchor similar to its attachment to the membrane of lymphocytes. Induction of dermatitis by topical application of PMA increased the expression of Ly-6.A2 on TCR gamma delta+ dendritic epidermal T cells and did not change its expression on keratinocytes. The increased expression of Ly-6.A2 on dendritic epidermal T cells was transient and reached a peak at 4 days after application of PMA, when 55% of the cells were positive.

摘要

我们通过流式细胞术研究了Ly-6.A2在分离的小鼠表皮细胞上的表达情况。在C57BL小鼠的61%的角质形成细胞和6%的树突状表皮T细胞上表达了Ly-6.A2。磷脂酰肌醇特异性磷脂酶C去除了Ly-6.A2，表明该抗原通过糖基磷脂酰肌醇锚定在角质形成细胞膜上，类似于其与淋巴细胞膜的附着方式。局部应用佛波酯诱导皮炎增加了Ly-6.A2在TCRγδ+树突状表皮T细胞上的表达，而在角质形成细胞上的表达没有变化。Ly-6.A2在树突状表皮T细胞上表达的增加是短暂的，在应用佛波酯后4天达到峰值，此时55%的细胞呈阳性。