Schaber B, Bruchelt G, Meyle J, Jeschke B, Handgretinger R, Niethammer D, Mayer P, Rassner G, Fierlbeck G
Department of Dermatology, Children's Hospital, Tuebingen, Germany.
Immunol Lett. 1994 Jun;41(1):67-71. doi: 10.1016/0165-2478(94)90058-2.
Chemotactic activity of granulocytes attracted by tumor cells loaded either with anti-ganglioside monoclonal antibodies (mAb) or with antibody-glucose oxidase conjugates (mAb-GO) was investigated. The melanoma cell line SK-Mel-28 which expresses the ganglioside GD3 at high density as well as the neuroectodermal cell line SK-N-LO which expresses GD2 were used for the experiments. In the presence of 50% human AB-serum, antibody-loaded tumor cells induced chemotactic activity on granulocytes, probably due to the generation of C3a/C5a which could be detected in serum incubated with anti-GD3 loaded SK-Mel-28 cells. Both compounds could also be detected in vivo in the plasma of patients suffering from neuroblastoma during therapy with anti-GD2 antibodies. In another set of experiments mAb-GO conjugates generating high amounts of H2O2 in the presence of glucose were bound to these tumor cells. A significant lipid peroxidation could be observed in the simultaneous presence of iron and ascorbate. The lipid peroxidation products were measured as thiobarbituric acid-reactive substances (TBARS) and were also shown to induce chemotactic effects on granulocytes.
研究了负载抗神经节苷脂单克隆抗体（mAb）或抗体-葡萄糖氧化酶缀合物（mAb-GO）的肿瘤细胞对粒细胞的趋化活性。实验使用了高密度表达神经节苷脂GD3的黑色素瘤细胞系SK-Mel-28以及表达GD2的神经外胚层细胞系SK-N-LO。在50%人AB血清存在的情况下，负载抗体的肿瘤细胞对粒细胞诱导了趋化活性，这可能是由于产生了C3a/C5a，在用负载抗GD3的SK-Mel-28细胞孵育的血清中可以检测到这两种物质。在患有神经母细胞瘤的患者接受抗GD2抗体治疗期间，也可以在其血浆中体内检测到这两种化合物。在另一组实验中，在葡萄糖存在下产生大量H2O2的mAb-GO缀合物与这些肿瘤细胞结合。在同时存在铁和抗坏血酸的情况下，可以观察到显著的脂质过氧化。脂质过氧化产物以硫代巴比妥酸反应性物质（TBARS）来测量，并且也显示出对粒细胞有趋化作用。
