Krishnan V V, Saraswathy A
Department of Pathology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
Indian J Exp Biol. 1994 Jun;32(6):405-8.
Experimental myositis was induced in guinea pigs by multiple inoculations with Myosin B fraction of the rabbit skeletal muscle. Quantitative histopathological features were studied and they include necrosis, central nucleation, inflammatory cellular reaction. Besides myositis, features of myocarditis were evident in 40% animals. Circulating immune complexes (CICs) in the sera of experimental animals were isolated by a polyethylene glycol (PEG) precipitation method. Presence of antimyosin B antibody in the immune complexes was characterised by an enzyme-linked immunosorbent assay. The role of circulating immune complexes in pathogenesis of experimental myositis and human polymyositis have been emphasised.
通过多次接种兔骨骼肌肌球蛋白B组分在豚鼠中诱导实验性肌炎。研究了定量组织病理学特征，包括坏死、中央核仁形成、炎症细胞反应。除了肌炎外，40%的动物有明显的心肌炎特征。通过聚乙二醇（PEG）沉淀法分离实验动物血清中的循环免疫复合物（CIC）。通过酶联免疫吸附测定法对免疫复合物中抗肌球蛋白B抗体的存在进行了表征。强调了循环免疫复合物在实验性肌炎和人类多发性肌炎发病机制中的作用。
