Sansonetti P J, Ryter A, Clerc P, Maurelli A T, Mounier J
Infect Immun. 1986 Feb;51(2):461-9. doi: 10.1128/iai.51.2.461-469.1986.
Virulent strains of Shigella flexneri invade HeLa cells with high efficiency. This crucial step in the pathogenic process is encoded by a 140-megadalton plasmid which induces phagocytosis of the bacteria by host cells. In this report we used pWR100, the virulence plasmid of S. flexneri serotype 5, and pHS4108, a 32-megadalton subclone of pWR100, to demonstrate that the plasmid is also responsible for rapid intracellular growth of the bacteria. The ability to replicate intracellularly was not correlated with induction of Shiga toxin. However, plasmid-mediated intracellular multiplication was strongly correlated with the ability of the bacteria to rapidly and efficiently lyse the phagocytic vacuole and replicate freely in the cytoplasm. Temperature-regulated plasmid-mediated contact hemolytic activity strongly correlated with both phagosomal membrane lysis and efficient intracellular multiplication. We propose this virulence plasmid-associated hemolysin to be an important factor in the invasion and proliferation of Shigella spp. in mammalian cells.
福氏志贺氏菌的强毒株能高效侵入HeLa细胞。致病过程中的这一关键步骤由一个140兆道尔顿的质粒编码，该质粒可诱导宿主细胞对细菌进行吞噬作用。在本报告中，我们使用了福氏志贺氏菌5型的毒力质粒pWR100以及pWR100的一个32兆道尔顿的亚克隆pHS4108，来证明该质粒也与细菌在细胞内的快速生长有关。在细胞内复制的能力与志贺毒素的诱导无关。然而，质粒介导的细胞内增殖与细菌快速有效地裂解吞噬泡并在细胞质中自由复制的能力密切相关。温度调节的质粒介导的接触溶血活性与吞噬体膜裂解和有效的细胞内增殖都密切相关。我们认为这种与毒力质粒相关的溶血素是志贺氏菌在哺乳动物细胞中侵袭和增殖的一个重要因素。
