Wróblewski R
Department of Pathology, Karolinska Institute, Stockholm, Sweden.
Histochemistry. 1994 Jul;101(6):405-12. doi: 10.1007/BF00269490.
The time between the excision and cryofixation of a biopsy is most important regarding its elemental composition as demonstrated by an investigation of the thyroid glands of rats and pigs. Biopsies taken and cryofixed immediately served as control specimens. Biopsies that were allowed to stand at room temperature for 20 min before cryofixation and specimens cryofixed at 1 h post mortem were also investigated. Significant changes in the ion concentration of the cells and colloid were apparent in biopsies in which cryofixation was delayed for 20 min and in thyroids cryofixed 1 h post mortem. It was demonstrated that redistribution of electrolytes occurs within 1h post mortem and that similar changes occur in biopsies allowed to stand for 20 min at room temperature before cryofixation. The results stress the importance of immediate cryofixation after surgical excision of a biopsy. This is especially important since numerous elemental changes due to delayed cryofixation resemble those which occur in pathological processes.
对大鼠和猪甲状腺的一项研究表明，活检组织切除与冷冻固定之间的时间对于其元素组成最为重要。立即取出并进行冷冻固定的活检组织用作对照标本。还对那些在冷冻固定前于室温下放置20分钟的活检组织以及死后1小时进行冷冻固定的标本进行了研究。在冷冻固定延迟20分钟的活检组织以及死后1小时进行冷冻固定的甲状腺中，细胞和胶体的离子浓度出现了显著变化。结果表明，电解质在死后1小时内会发生重新分布，并且在冷冻固定前于室温下放置20分钟的活检组织中也会出现类似变化。这些结果强调了活检手术切除后立即进行冷冻固定的重要性。这一点尤为重要，因为冷冻固定延迟导致的众多元素变化类似于病理过程中发生的变化。
