Roomans G M, Wroblewski J
Scan Electron Microsc. 1985(Pt 2):681-6.
Possible alternatives to rapid freezing in liquid nitrogen of tissue for X-ray microanalysis of electrolytes at the cellular level were investigated. These alternatives might be used in cases where tissue becomes available for examination, e.g., at autopsy, but liquid nitrogen is not immediately available. Rat submandibular gland was used as a test tissue. Freezing of pieces of tissue in a conventional freezer at -80 degrees C or even at -20 degrees C retained the elemental distribution at the cellular level, and also retained the difference between a 'normal' and a 'pathological' (mimicked by an injection of a high dose of isoproterenol) situation. Storage of tissue in a refrigerator, or delaying the autopsy in anticipation of the arrival of liquid nitrogen is not recommended. Significant changes in the cellular ion content occurred if the tissue was left in the animal for 24h post-mortem.
研究了在细胞水平对组织电解质进行X射线微量分析时，液氮快速冷冻组织的可能替代方法。这些替代方法可用于在组织可供检查的情况下，例如尸检时，但液氮并非立即可用。大鼠下颌下腺用作测试组织。在传统冰箱中于-80℃甚至-20℃冷冻组织块，可在细胞水平保留元素分布，并且还保留了“正常”与“病理”（通过注射高剂量异丙肾上腺素模拟）情况之间的差异。不建议将组织储存在冰箱中，或因等待液氮到来而延迟尸检。如果组织在动物死后留存24小时，细胞离子含量会发生显著变化。
