Inostroza J, Muñoz P, Espinoza R, Millaqueo L, Diaz P, Leiva L, Sorensen R
Immunology Laboratory, Hospital de Temuco, Chile.
Hum Immunol. 1994 Jul;40(3):179-82. doi: 10.1016/0198-8859(94)90066-3.
Beta 2m serum levels have been shown to be increased in patients with tuberculosis and HIV infection. We determined the stability of beta 2m and of sHLA-I dimers in serum, and then determined the levels of both molecules in 60 non-HIV-infected patients with active pulmonary tuberculosis and in 55 adult controls. The levels of sHLA-I in samples kept at room temperature declined by 8% at 30 minutes, 16% at 60 minutes, and 36% at 120 minutes. Beta 2m levels remained stable at all times tested. Mean sHLA-I levels were 0.99 +/- 0.16 micrograms/ml in controls and 1.34 +/- 0.11 micrograms/ml in patients with tuberculosis (P < 0.0001). Beta 2m levels were 1.23 +/- 0.26 micrograms/ml in controls and 2.26 +/- 0.64 micrograms/ml in patients with tuberculosis (P < 0.0001). All patients with tuberculosis had elevation of sHLA-I and/or beta 2m above 1 standard deviation of normal values. However, there was no correlation between sHLA-I and beta 2m levels in individual samples. Evaluation of sHLA-I holds the promise of further understanding of the biology and genetic regulation of the immune response to mycobacterial infection.
已有研究表明，结核病患者和艾滋病毒感染者的β2m血清水平会升高。我们测定了血清中β2m和可溶性 HLA-I 二聚体的稳定性，然后测定了60例活动性肺结核非艾滋病毒感染患者和55例成人对照组中这两种分子的水平。室温下保存的样本中，可溶性 HLA-I 水平在30分钟时下降了8%，60分钟时下降了16%，120分钟时下降了36%。β2m水平在所有测试时间均保持稳定。对照组的平均可溶性 HLA-I 水平为0.99±0.16微克/毫升，结核病患者为1.34±0.11微克/毫升（P<0.0001）。对照组的β2m水平为1.23±0.26微克/毫升，结核病患者为2.26±0.64微克/毫升（P<0.0001）。所有结核病患者的可溶性 HLA-I 和/或β2m水平均高于正常值1个标准差。然而，单个样本中可溶性 HLA-I 和β2m水平之间没有相关性。对可溶性 HLA-I 的评估有望进一步了解对分枝杆菌感染免疫反应的生物学和基因调控。
