Ohtsuka H, Mafune Y, Tsunashima K, Takahashi H, Kominami R
First Department of Biochemistry, Niigata University School of Medicine.
Jpn J Cancer Res. 1994 Oct;85(10):1015-22. doi: 10.1111/j.1349-7006.1994.tb02899.x.
Allelic expression was examined by single-strand conformation polymorphism analysis in murine fibrosarcomas from inter-subspecific F1 mice between C57BL/6 and MSM. Ten genes encoding p53, mdm2, E-cadherin, 72 kD metalloproteinase and its inhibitor (Timp2), thymidine kinase and four glucose transporters (Gluts) were examined. These genes were chosen because of their probable association with tumor development and progression. In some of the tumors and cell lines, p53, E-cadherin and Glut3 genes showed remarkable differences in allelic expression, one allele being poorly expressed. The allele-specificity persisted in nine cell lines obtained by repeated transplantations from one tumor. These results suggest that expression of some genes is allele-specific in tumor cells and the pattern of specificity is stable. Such a decrease or a loss of expression in one of the alleles may be functionally equivalent to the loss of heterozygosity of the gene, and therefore this may confer malignant properties on tumor cells. It is also suggested that differential expression of two alleles is a common event in tumor cells.
通过单链构象多态性分析，检测了C57BL/6和MSM之间亚种间F1小鼠的鼠纤维肉瘤中的等位基因表达。检测了十个基因，分别编码p53、mdm2、E-钙黏蛋白、72 kD金属蛋白酶及其抑制剂（Timp2）、胸苷激酶和四种葡萄糖转运蛋白（Gluts）。选择这些基因是因为它们可能与肿瘤的发生和发展有关。在一些肿瘤和细胞系中，p53、E-钙黏蛋白和Glut3基因在等位基因表达上表现出显著差异，其中一个等位基因表达较差。通过从一个肿瘤反复传代获得的九个细胞系中，等位基因特异性持续存在。这些结果表明，某些基因在肿瘤细胞中的表达具有等位基因特异性，且特异性模式是稳定的。一个等位基因表达的降低或丧失在功能上可能等同于该基因杂合性的丧失，因此这可能赋予肿瘤细胞恶性特性。还表明两个等位基因的差异表达在肿瘤细胞中是常见事件。
