Saito Y, Kosugi S, Suda T, Wakabayashi Y, Mishima Y, Hatakeyama K, Kominami R
First Department of Biochemistry, Niigata University School of Medicine.
Jpn J Cancer Res. 1997 Aug;88(8):732-7. doi: 10.1111/j.1349-7006.1997.tb00444.x.
Tumor cells with metastatic potential may have a high telomerase activity that augments telomeric DNA repeats, allowing the cells to escape from the inhibition of cell proliferation due to shortened telomeres. We examined the expression level of telomerase activity using the telomeric repeat amplification protocol among a series of cell lines obtained by repeated transplantation of a mouse fibrosarcoma. The lines could be grouped into three; one has no metastatic potential, and the other two show metastatic abilities after intravenous or subcutaneous injection. Comparison of their telomerase activity indicated that more malignant lines had higher activity. A similar relation was seen in metastatic nodules formed through clonal expansion from the heterogeneous population of inoculated cells; clonality was monitored in terms of variable patterns of subtelomeric repeats. The results suggest that a high level of telomerase activity may not be requisite for metastasis, but may confer a propensity to dominate in a tumor tissue.
具有转移潜能的肿瘤细胞可能具有较高的端粒酶活性，这种活性会增加端粒DNA重复序列，使细胞能够逃避因端粒缩短而导致的细胞增殖抑制。我们使用端粒重复序列扩增法检测了一系列通过小鼠纤维肉瘤反复移植获得的细胞系中端粒酶活性的表达水平。这些细胞系可分为三组；一组没有转移潜能，另外两组在静脉注射或皮下注射后显示出转移能力。对它们端粒酶活性的比较表明，恶性程度更高的细胞系具有更高的活性。在通过接种细胞的异质群体克隆扩增形成的转移结节中也观察到了类似的关系；根据亚端粒重复序列的可变模式监测克隆性。结果表明，高水平的端粒酶活性可能不是转移所必需的，但可能赋予在肿瘤组织中占主导地位的倾向。
