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Molecular cloning and characterization of the aklavinone 11-hydroxylase gene of Streptomyces peucetius subsp. caesius ATCC 27952.

作者信息

Hong Y S, Hwang C K, Hong S K, Kim Y H, Lee J J

机构信息

Genetic Engineering Research Institute, Korea Institute of Science and Technology, Taejon.

出版信息

J Bacteriol. 1994 Nov;176(22):7096-101. doi: 10.1128/jb.176.22.7096-7101.1994.

DOI:10.1128/jb.176.22.7096-7101.1994
PMID:7961477
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC197087/
Abstract

The gene encoding aklavinone 11-hydroxylase of Streptomyces peucetius subsp. caesius ATCC 27952 was cloned and sequenced. The deduced amino acid sequence of the gene contains at least two common motifs of well-conserved amino acid sequences of several flavin-type bacterial hydroxylases. The hydroxylase gene is apparently transcribed from a single transcriptional start point. The phenotype of a dnrF mutant generated by gene disruption supports the idea that the dnrF gene encodes aklavinone 11-hydroxylase.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/263f/197087/e8e7220dff57/jbacter00040-0326-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/263f/197087/a14c62cb0496/jbacter00040-0325-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/263f/197087/e8e7220dff57/jbacter00040-0326-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/263f/197087/a14c62cb0496/jbacter00040-0325-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/263f/197087/e8e7220dff57/jbacter00040-0326-a.jpg

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本文引用的文献

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Cloning and sequencing of a gene encoding carminomycin 4-O-methyltransferase from Streptomyces peucetius and its expression in Escherichia coli.来自产红链霉菌的一种编码洋红霉素4-O-甲基转移酶的基因的克隆、测序及其在大肠杆菌中的表达。
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Nucleotide sequences and heterologous expression of tcmG and tcmP, biosynthetic genes for tetracenomycin C in Streptomyces glaucescens.青灰链霉菌中四环素霉素C生物合成基因tcmG和tcmP的核苷酸序列及异源表达
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A hydroxylase-like gene product contributes to synthesis of a polyketide spore pigment in Streptomyces halstedii.
Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tü2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation.
来自弗氏链霉菌Tü2717的聚酮合酶基因的克隆与特性分析，该菌株携带安古环素类抗生素乌达霉素A的生物合成基因以及一个可能参与其氧化过程的基因。
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Expression of Streptomyces peucetius genes for doxorubicin resistance and aklavinone 11-hydroxylase in Streptomyces galilaeus ATCC 31133 and production of a hybrid aclacinomycin.在加利利链霉菌ATCC 31133中表达产多柔比星抗性的天蓝色链霉菌基因和阿克拉酮11-羟化酶，并产生一种杂交阿克拉霉素。
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一种类似羟化酶的基因产物有助于哈氏链霉菌中聚酮类孢子色素的合成。
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