Ho A M, Jain J, Rao A, Hogan P G
Department of Neurobiology, Harvard Medical School, Boston, Massachusetts.
J Biol Chem. 1994 Nov 11;269(45):28181-6.
The transcription factor NFATp, which has a central role in cytokine gene induction in T cells, was previously thought to be expressed only in T cells and other immune cells. Here we report that NFATp is also expressed in the rat PC12 pheochromocytoma cell line and in the murine nervous system. Antisera against T cell NFATp recognize proteins of similar apparent molecular weight in T cells, PC12 cells, and murine olfactory bulb. The immunoreactive proteins from PC12 cells and from olfactory bulb bind to an NFAT site of the murine interleukin-2 promoter with the same specificity as T cell NFATp. Immunohistochemical staining localizes NFATp to neurons, specifically to olfactory receptor neurons and to their terminals in the olfactory bulb. The presence of NFATp in the nervous system suggests that it has a role in the transcription of specific neuronal genes in response to increases in cytosolic calcium.
转录因子NFATp在T细胞中细胞因子基因诱导过程中起核心作用，以前认为它仅在T细胞和其他免疫细胞中表达。在此我们报告，NFATp也在大鼠PC12嗜铬细胞瘤细胞系和小鼠神经系统中表达。针对T细胞NFATp的抗血清能识别T细胞、PC12细胞和小鼠嗅球中表观分子量相似的蛋白质。来自PC12细胞和嗅球的免疫反应性蛋白以与T细胞NFATp相同的特异性结合到小鼠白细胞介素-2启动子的NFAT位点。免疫组织化学染色将NFATp定位于神经元，特别是嗅受体神经元及其在嗅球中的终末。NFATp在神经系统中的存在表明它在响应胞质钙增加时对特定神经元基因的转录起作用。
