Ferrari S, Ronfani L, Calogero S, Bianchi M E
DIBIT, San Raffaele Scientific Institute, Milano, Italy.
J Biol Chem. 1994 Nov 18;269(46):28803-8.
We have isolated an active gene encoding the mouse HMG1 protein among a multitude of cross-hybridizing sequences, which most likely are retrotransposed pseudogenes. The hmg1 gene contains five exons, of which the first is not translated, and the last contains a long 3'-untranslated sequence and three alternative polyadenylation sites. We found no evidence for a sequence encoding a membrane localization signal in the hmg1 gene, despite the presence of HMG1 protein on the surface of several cell types. The hmg1 promoter coincides with a CpG island, contains no TATA sequence, and derives the expression of reporter genes placed under its control. The hmg1 gene may be a member of a family of closely related genes but appears to be the major or the only active gene coding for HMG1 protein.
我们在众多交叉杂交序列中分离出了一个编码小鼠HMG1蛋白的活性基因，这些交叉杂交序列很可能是反转录转座假基因。hmg1基因包含五个外显子，其中第一个不进行翻译，最后一个包含一个长的3'非翻译序列和三个可变聚腺苷酸化位点。尽管在几种细胞类型的表面存在HMG1蛋白，但我们在hmg1基因中未发现编码膜定位信号的序列证据。hmg1启动子与一个CpG岛重合，不包含TATA序列，并驱动置于其控制下的报告基因的表达。hmg1基因可能是一个紧密相关基因家族的成员，但似乎是编码HMG1蛋白的主要或唯一活性基因。
