Stevenson S, Cunningham N, Toth J, Davy D, Reddi A H
Department of Orthopaedics, Case Western Reserve University, Cleveland, Ohio 44106.
J Bone Joint Surg Am. 1994 Nov;76(11):1676-87. doi: 10.2106/00004623-199411000-00011.
We studied the effects of partially purified, natural osteogenin, a bone morphogenetic protein, on the formation of bone in rats. An osteoperiosteal segmental defect, eight millimeters wide, in the middle of the femoral diaphysis was created bilaterally in thirty-six adult male Fischer rats and stabilized with a polyacetyl plate and threaded Kirschner wires. One defect was filled with a cylinder of 60 per cent hydroxyapatite and 40 per cent tricalcium phosphate ceramic (pore diameter, 250 to 400 micrometers) containing 100 micrograms of partially purified bovine osteogenin, and the contralateral defect was filled with a hydroxyapatite-tricalcium ceramic cylinder without osteogenin. Eighteen animals (six animals each at one, two, and four months after the operation) were studied histologically and histomorphometrically. The implants from eighteen additional animals (six animals each at one, two, and four months after the operation) were subjected to biomechanical testing. Histomorphometry revealed that the total area of bone, the area of bone outside of the implant, and the amount of bone within the pores of the implant were all significantly (p < or = 0.05) greater in the femora that had an implant with osteogenin than in those that had an implant without osteogenin at most time-periods. The presence of osteogenin had no significant effect on the biomechanical parameters measured in this study.
我们研究了部分纯化的天然骨生成素（一种骨形态发生蛋白）对大鼠骨形成的影响。在36只成年雄性Fischer大鼠双侧股骨骨干中部制造一个8毫米宽的骨膜骨节段性缺损，并用聚乙酰板和带螺纹的克氏针进行固定。一个缺损处填充一个含有100微克部分纯化牛骨生成素的60%羟基磷灰石和40%磷酸三钙陶瓷圆柱体（孔径250至400微米），对侧缺损处填充一个不含骨生成素的羟基磷灰石 - 磷酸三钙陶瓷圆柱体。对18只动物（术后1个月、2个月和4个月各6只）进行组织学和组织形态计量学研究。对另外18只动物（术后1个月、2个月和4个月各6只）的植入物进行生物力学测试。组织形态计量学显示，在大多数时间段，植入含骨生成素的股骨中，骨的总面积、植入物外的骨面积以及植入物孔隙内的骨量均显著（p≤0.05）大于植入不含骨生成素的股骨。骨生成素的存在对本研究中测量的生物力学参数没有显著影响。
