Tisdel C L, Goldberg V M, Parr J A, Bensusan J S, Staikoff L S, Stevenson S
Department of Orthopaedics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
J Bone Joint Surg Am. 1994 Feb;76(2):159-71. doi: 10.2106/00004623-199402000-00001.
A study was done in rabbits to determine the effect of a hydroxyapatite and tricalcium-phosphate coating on bone growth into titanium fiber-metal implants. Titanium fiber rods with a solid titanium core were implanted bilaterally into the distal aspect of the femora of fifty-five New Zealand White rabbits. One rod was uncoated and the other rod was surface-coated with hydroxyapatite and tricalcium phosphate by the plasma-spray technique. Thirty-five rabbits were labeled sequentially with fluorochromes; killed at one, two, three, four, six, twelve, or twenty-four weeks after the operation; and studied histologically and histomorphometrically. The implants in the remaining twenty rabbits were subjected to pull-out testing to determine the shear strength at the implant-bone interface at three, six, twelve, and twenty-four weeks after the operation. Histomorphometry revealed significant effects of the hydroxyapatite and tricalcium-phosphate coating. When whole-group means (which included all time-points) were compared, it was found that 44 per cent of the perimeter of the hydroxyapatite and tricalcium-phosphate-coated implants was covered with bone compared with 12 per cent of the perimeter of the uncoated implants. The percentage of the internal surface of the implant that was covered with bone was also significantly higher in the hydroxyapatite and tricalcium-phosphate-coated implants: 27 per cent of the internal surface of the coated implants was covered compared with 8 per cent in the uncoated implants. The amount of bone in the pores of the implants was also higher in the hydroxyapatite and tricalcium-phosphate-coated implants: 12 per cent of the available pore space in the hydroxyapatite and tricalcium-phosphate-coated implants was filled with bone compared with 4 per cent in the uncoated implants. Scanning electron microscopy of the implants, done in backscatter mode, demonstrated apposition of new bone directly on the hydroxyapatite and tricalcium-phosphate coating, with variable degrees (amounts) of hydroxyapatite and tricalcium-phosphate resorption and new-bone replacement over time. Bone was never directly apposed to uncoated titanium fiber-metal. The pull-out strength of the hydroxyapatite and tricalcium-phosphate-coated implants was consistently greater than that of the uncoated implants, at all time-periods.
在兔子身上进行了一项研究，以确定羟基磷灰石和磷酸三钙涂层对骨长入钛纤维金属植入物的影响。将具有实心钛芯的钛纤维棒双侧植入55只新西兰白兔股骨的远端。一根棒未涂层，另一根棒通过等离子喷涂技术表面涂覆有羟基磷灰石和磷酸三钙。35只兔子依次用荧光染料标记；在手术后1、2、3、4、6、12或24周处死；并进行组织学和组织形态计量学研究。对其余20只兔子的植入物进行拔出试验，以确定手术后3、6、12和24周时植入物与骨界面处的剪切强度。组织形态计量学显示羟基磷灰石和磷酸三钙涂层有显著影响。当比较全组平均值（包括所有时间点）时，发现羟基磷灰石和磷酸三钙涂层植入物周长的44%被骨覆盖，而未涂层植入物周长的这一比例为12%。在羟基磷灰石和磷酸三钙涂层植入物中，植入物内表面被骨覆盖的百分比也显著更高：涂层植入物内表面的27%被骨覆盖，而未涂层植入物为8%。羟基磷灰石和磷酸三钙涂层植入物中植入物孔隙内的骨量也更高：羟基磷灰石和磷酸三钙涂层植入物中可用孔隙空间的12%被骨填充，而未涂层植入物为4%。以背散射模式对植入物进行扫描电子显微镜检查，结果表明新骨直接附着在羟基磷灰石和磷酸三钙涂层上，随着时间的推移，羟基磷灰石和磷酸三钙有不同程度（数量）的吸收和新骨替代。骨从未直接附着在未涂层的钛纤维金属上。在所有时间段，羟基磷灰石和磷酸三钙涂层植入物的拔出强度始终大于未涂层植入物。
