多核糖体与微管的靶向作用：海胆胚胎重构微管制剂中特定mRNA的富集
Polyribosome targeting to microtubules: enrichment of specific mRNAs in a reconstituted microtubule preparation from sea urchin embryos.
作者信息
Hamill D, Davis J, Drawbridge J, Suprenant K A
机构信息
Department of Physiology and Cell Biology, University of Kansas, Lawrence 66045.
出版信息
J Cell Biol. 1994 Nov;127(4):973-84. doi: 10.1083/jcb.127.4.973.
Abstract
A subset of mRNAs, polyribosomes, and poly(A)-binding proteins copurify with microtubules from sea urchin embryos. Several lines of evidence indicate that the interaction of microtubules with ribosomes is specific: a distinct stalk-like structure appears to mediate their association; ribosomes bind to microtubules with a constant stoichiometry through several purification cycles; and the presence of ribosomes in these preparations depends on the presence of intact microtubules. Five specific mRNAs are enriched with the microtubule-bound ribosomes, indicating that translation of specific proteins may occur on the microtubule scaffolding in vivo.
摘要
来自海胆胚胎的一部分信使核糖核酸（mRNA）、多核糖体和聚腺苷酸结合蛋白能与微管共同纯化。几条证据表明微管与核糖体之间的相互作用是特异性的：一种独特的茎状结构似乎介导了它们的结合；核糖体通过几个纯化循环以恒定的化学计量比与微管结合；并且这些制剂中核糖体的存在取决于完整微管的存在。五种特定的mRNA在与微管结合的核糖体中富集，这表明特定蛋白质的翻译可能在体内的微管支架上发生。
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