通过动态显色鲎试剂法检测组织培养基中皮克级的脂多糖。

Detection of lipopolysaccharide in the picogram range of tissue culture media by a kinetic chromogenic Limulus amebocyte lysate assay.

作者信息

Pedersen M R, Hansen E W, Christensen J D

机构信息

Royal Danish School of Pharmacy, Department of Biological Sciences, Copenhagen.

出版信息

J Clin Pharm Ther. 1994 Jun;19(3):189-94. doi: 10.1111/j.1365-2710.1994.tb00671.x.

DOI:10.1111/j.1365-2710.1994.tb00671.x

PMID:7962222

Abstract

A kinetic chromogenic Limulus amebocyte lysate assay for detection of lipopolysaccharide in the picogram range is described. The kinetic Limulus assay was used to detect lipopolysaccharide in a tissue culture media containing interfering low-molecular-weight substances. The interfering substances were removed by ultrafiltration. The recovery of lipopolysaccharide from the ultrafiltrated tissue culture media was 61.6 +/- 21.4%. The kinetic Limulus assay was compared with the traditional end-point chromogenic Limulus assay. The results in the two assays showed a good correlation. The detection limit was 1 pg/ml in the chromogenic assay and 0.25 pg/ml in the kinetic assay.

摘要

描述了一种用于检测皮克级脂多糖的动力学显色鲎试剂检测法。动力学鲎试剂检测法用于检测含有干扰性低分子量物质的组织培养基中的脂多糖。通过超滤去除干扰物质。从超滤后的组织培养基中回收脂多糖的回收率为61.6±21.4%。将动力学鲎试剂检测法与传统的终点显色鲎试剂检测法进行比较。两种检测法的结果显示出良好的相关性。显色检测法的检测限为1 pg/ml，动力学检测法的检测限为0.25 pg/ml。