Sonesson A, Larsson L, Schütz A, Hagmar L, Hallberg T
Department of Technical Analytical Chemistry, Lund University, Sweden.
Appl Environ Microbiol. 1990 May;56(5):1271-8. doi: 10.1128/aem.56.5.1271-1278.1990.
The lipopolysaccharide (endotoxin) content in airborne dust samples from three different poultry slaughterhouses was determined with both the chromogenic Limulus amebocyte lysate assay and gas chromatography-mass spectrometry analysis of lipopolysaccharide-derived 3-hydroxy fatty acids. Gram-negative cell walls were also measured by using two-dimensional gas chromatography/electron-capture analysis of diaminopimelic acid originating from the peptidoglycan. The correlation between the results of the Limulus assay and those of gas chromatography-mass spectrometry for determination of the lipopolysaccharide content in the dust samples was poor, whereas a good correlation was obtained between lipopolysaccharide and diaminopimelic acid concentrations with the gas chromatographic methods. The results suggest that it is predominantly cell-wall-dissociated lipopolysaccharides that are measured with the Limulus assay, whereas the gas chromatographic methods allow determination of total concentrations of lipopolysaccharide, including Limulus-inactive lipopolysaccharide, gram-negative cells, and cellular debris.
采用显色鲎试剂法以及对脂多糖衍生的3-羟基脂肪酸进行气相色谱-质谱分析，测定了来自三家不同家禽屠宰场的空气尘埃样本中的脂多糖（内毒素）含量。还通过对源自肽聚糖的二氨基庚二酸进行二维气相色谱/电子捕获分析来测定革兰氏阴性菌细胞壁。鲎试剂法与气相色谱-质谱法测定尘埃样本中脂多糖含量的结果之间相关性较差，而采用气相色谱法时，脂多糖与二氨基庚二酸浓度之间具有良好的相关性。结果表明，鲎试剂法主要测定的是细胞壁解离的脂多糖，而气相色谱法能够测定脂多糖的总浓度，包括对鲎试剂无反应的脂多糖、革兰氏阴性菌细胞以及细胞碎片。
