禽呼肠孤病毒野毒株双链RNA基因组片段分析

Analysis of the double-stranded RNA genome segments among avian reovirus field isolates.

作者信息

Wu W Y, Shien J H, Lee L H, Shieh H K

机构信息

Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan.

出版信息

J Virol Methods. 1994 Jun;48(1):119-22. doi: 10.1016/0166-0934(94)90094-9.

DOI:10.1016/0166-0934(94)90094-9

PMID:7962256

Abstract

Nine isolates of avian reovirus (ARV) from both healthy birds and birds with different clinical illness and one commercially available vaccine strain were selected and characterized by analysis of the migration pattern of their genomic double-stranded RNA (dsRNA) segments following separation by polyacrylamide gel electrophoresis. Different electropherotypes were observed and analyzed. The results show that the dsRNA segments of ARV were markedly polymorphic among isolates within the same serotype as well as among different serotypes. The results also show no correlation between electropherotype and disease state.

摘要

从健康鸟类和患有不同临床疾病的鸟类中选取了9株禽呼肠孤病毒（ARV）分离株以及1株市售疫苗株，并通过分析其基因组双链RNA（dsRNA）片段在聚丙烯酰胺凝胶电泳分离后的迁移模式进行了鉴定。观察并分析了不同的电泳图谱型。结果表明，ARV的dsRNA片段在同一血清型的分离株之间以及不同血清型之间均具有明显的多态性。结果还表明，电泳图谱型与疾病状态之间没有相关性。