Omi K, Kitano Y, Agawa H, Kadota K
Institute of Animal Health, Gunma Prefecture, Japan.
J Comp Pathol. 1994 Jul;111(1):1-14. doi: 10.1016/s0021-9975(05)80106-9.
Immunohistochemical analysis of five paraffin wax-embedded neoplasms was performed to elucidate the characteristics of bovine nervous-tissue tumours. In case 1 (peripheral neuroblastoma), the neoplastic tissue was characterized by the formation of true and Homer-Wright rosettes and the existence of neuron-specific enolase. The neoplastic cells were possibly more immature than those of common neuroblastomas, because similar features are observed in human malignant neuroepitheliomas. The neoplastic cells in case 2 (ganglioneuroblastoma) ranged from large cells with abundant neurofilaments to immature small cells, rarely with neurofilaments or glial fibrillary acidic protein (GFAP). Such expression suggests the presence of pluripotential cells. The neoplastic tissue in case 3 (anaplastic ganglioglioma) was strikingly polymorphous, and had five elements; neuronal, astrocytic, oligodendrocytic, spindle cell and small oval cell. The neoplastic neurocytes and astrocytes were, respectively, characterized by neurofilament and GFAP positivity. The neoplastic oligodendrocytes made a honeycomb appearance, and the neoplastic spindle cells and small oval cells were considered to be less differentiated. The tumours of cases 2 and 3, which contained poorly differentiated cells and revealed both neuronal and glial differentiation, may be specific to calves. In case 4 (schwannoma), almost all the neoplastic cells were positive for S100 protein, while S100-negative fibroblasts were present in many areas of case 5 (neurofibroma). These two tumours were readily distinguished histologically and immunohistochemically.
对五个石蜡包埋的肿瘤进行免疫组织化学分析，以阐明牛神经组织肿瘤的特征。病例1（外周神经母细胞瘤）中，肿瘤组织的特征是形成真性和霍纳-赖特菊形团以及存在神经元特异性烯醇化酶。肿瘤细胞可能比普通神经母细胞瘤的细胞更不成熟，因为在人类恶性神经上皮瘤中也观察到类似特征。病例2（节细胞神经母细胞瘤）中的肿瘤细胞范围从具有丰富神经丝的大细胞到不成熟的小细胞，很少有神经丝或胶质纤维酸性蛋白（GFAP）。这种表达提示存在多能细胞。病例3（间变性节细胞胶质瘤）的肿瘤组织具有显著的多形性，有五种成分；神经元、星形胶质细胞、少突胶质细胞、梭形细胞和小卵圆形细胞。肿瘤性神经细胞和星形胶质细胞分别以神经丝和GFAP阳性为特征。肿瘤性少突胶质细胞呈蜂窝状外观，肿瘤性梭形细胞和小卵圆形细胞被认为分化程度较低。病例2和3的肿瘤含有低分化细胞，并显示出神经元和神经胶质分化，可能是犊牛特有的。病例4（施万细胞瘤）中，几乎所有肿瘤细胞S100蛋白呈阳性，而病例5（神经纤维瘤）的许多区域存在S100阴性的成纤维细胞。这两种肿瘤在组织学和免疫组织化学上很容易区分。
