Chaisiri N, Valotaire Y, Evans B A, Pierrepoint C G
J Endocrinol. 1978 Jul;78(1):131-9. doi: 10.1677/joe.0.0780131.
A receptor protein that selectively binds oestrogens has been demonstrated in the cytosol of the canine prostate gland. The steroid--receptor complex was found to have a sedimentation coefficient of 4--5 S with respect to bovine serum albumin after sucrose density-gradient centrifugation. The high affinity and low capacity of the protein for oestrogens was indicated by displacement studies, which gave a value of 3.8 +/- 1.53 (S.D.) X 10(-10) mol/l for the dissociation constant. A metastasizing prostatic tumour was also shown to possess this receptor, with binding properties similar to those exhibited by the receptor in normal prostatic cytosol. The implications of these findings are discussed with regard to normal prostatic function in the dog and the virtually inevitable advent of prostatic hyperplasia with age in this species.
在犬前列腺的胞质溶胶中已证实有一种能选择性结合雌激素的受体蛋白。经蔗糖密度梯度离心后，相对于牛血清白蛋白，该类固醇 - 受体复合物的沉降系数为4 - 5 S。置换研究表明该蛋白对雌激素具有高亲和力和低容量，解离常数的值为3.8 +/- 1.53（标准差）×10^(-10) mol/l。还显示一个转移性前列腺肿瘤也具有这种受体，其结合特性与正常前列腺胞质溶胶中的受体相似。讨论了这些发现对于犬正常前列腺功能以及该物种中随着年龄增长几乎不可避免出现的前列腺增生的意义。
