Jarvis W R
Investigation and Prevention Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333.
Infect Control Hosp Epidemiol. 1994 Jul;15(7):500-3. doi: 10.1086/646959.
We conducted a retrospective review of nosocomial outbreak investigations conducted by the Hospital Infections Program, Centers for Disease Control and Prevention, from January 1991 through March 1994. Selected outbreaks have demonstrated the utility of molecular methods such as plasmid analysis, plasmid restriction endonuclease analysis, ribotyping, restriction fragment polymorphism, pulsed-field gel electrophoresis, and polymerase chain reaction in confirming the clonality of the outbreak and in confirming the source of the outbreak implicated in the epidemiologic investigation. These data show that molecular typing of isolates is particularly useful when combined with epidemiologic investigations of nosocomial outbreaks.
我们对疾病控制和预防中心医院感染项目在1991年1月至1994年3月期间进行的医院感染暴发调查进行了回顾性研究。部分暴发事件已证明分子方法的实用性，如质粒分析、质粒限制性内切酶分析、核糖体分型、限制性片段多态性、脉冲场凝胶电泳和聚合酶链反应，这些方法有助于确认暴发的克隆性，并确认流行病学调查中所涉及的暴发源头。这些数据表明，分离株的分子分型与医院感染暴发的流行病学调查相结合时特别有用。
