Branchini M L, Morthland V H, Tresoldi A T, Von Nowakonsky A, Dias M B, Pfaller M A
Study of Transmitted Diseases, Campinas State University, São Paulo, Brazil.
Diagn Microbiol Infect Dis. 1993 Nov-Dec;17(4):275-81. doi: 10.1016/0732-8893(93)90036-7.
Pulsed-field gel electrophoresis (PFGE) and restriction enzyme analysis of plasmid DNA (REAP) were applied to study the epidemiologic relationship among methicillin-resistant Staphylococcus aureus (MRSA) isolates from outbreaks in two hospitals in São Paulo, Brazil: 82 MRSA isolates, 73 from a university hospital and nine from a general adult intensive care unit of a private hospital, were collected from 62 patients: 95% of the MRSAs were also resistant to gentamicin and ciprofloxacin. REAP subtyping of both collections identified six different subtypes: 55 (72.6%) MRSAs from the university hospital and nine isolates from the private hospital shared the same epidemic REAP subtype. Discrimination by restriction of genomic DNA with Sma I followed by PFGE enabled the identification of 14 DNA subtypes. Based on the combined REAP-genomic DNA subtype, the predominant subtype in the university hospital was A/A (44 isolates) whereas the epidemic subtype in the private hospital was A/M (seven isolates). The application of two typing methods showed better discrimination among MRSAs than did either method alone.
采用脉冲场凝胶电泳（PFGE）和质粒DNA限制性酶切分析（REAP）来研究巴西圣保罗两家医院耐甲氧西林金黄色葡萄球菌（MRSA）暴发菌株之间的流行病学关系：从62例患者中收集了82株MRSA菌株，其中73株来自一家大学医院，9株来自一家私立医院的成人重症监护病房；95%的MRSA菌株同时对庆大霉素和环丙沙星耐药。对两组菌株进行REAP分型，鉴定出六种不同的亚型：大学医院的55株（72.6%）MRSA菌株和私立医院的9株菌株具有相同的流行REAP亚型。先用Sma I对基因组DNA进行酶切，再进行PFGE，可鉴定出14种DNA亚型。根据REAP-基因组DNA联合亚型，大学医院的主要亚型为A/A（44株），而私立医院的流行亚型为A/M（7株）。两种分型方法的应用比单独使用任何一种方法对MRSA菌株的鉴别能力更强。
