Fenjves E S
Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York, Stony Brook 11794-8702.
J Invest Dermatol. 1994 Nov;103(5 Suppl):70S-75S. doi: 10.1111/1523-1747.ep12399089.
The introduction and expression of exogenous genetic material in cultured cells has provided a powerful tool for studying gene function and regulation. Immortalized cell lines have been useful for establishing gene transfer methodologies that are generally inefficient. For investigators of epidermal and mucosal biology, wishing to make use of the tissue architecture produced by primary keratinocytes in vitro, the limited life span of these cells presents a host of unique problems. Primary cells require the use of gene transfer methods that are highly efficient and will not significantly alter the cell's normal differentiation pathway. The purpose of this review is to evaluate gene transfer technology as it applies to keratinocytes.
外源遗传物质在培养细胞中的导入与表达为研究基因功能和调控提供了强大工具。永生化细胞系对于建立通常效率不高的基因转移方法很有用。对于希望利用原代表皮角质形成细胞在体外形成的组织结构来研究表皮和黏膜生物学的研究者而言，这些细胞有限的寿命带来了一系列独特问题。原代细胞需要使用高效且不会显著改变细胞正常分化途径的基因转移方法。本综述的目的是评估适用于角质形成细胞的基因转移技术。
