Suzuki N, Ueda Y, Mori H, Miyagi K, Noda K, Hirose H, Oosumi Y, Ishibashi M, Yoh M, Yamamoto K
Osaka Airport Quarantine Station.
Kansenshogaku Zasshi. 1994 Sep;68(9):1068-74. doi: 10.11150/kansenshogakuzasshi1970.68.1068.
We analysed 467 isolates of Vibrio parahaemolyticus for possession of tdh/trh gene in comparison with urease production and serotypes. Strains possessing tdh+/trh-, tdh+/trh+, tdh-/trh-, and tdh-/trh- show positive urease production 2.1, 100, 65.7, 100%, respectively. Serotypes of O1:K69, O3:K6, O3:K72, O6:K18, O6:K46 and O1:KUT were frequently positive (100% except 91.7% of the latest one) in urease production. All isolates of O3:K6 prossessed trh, whereas all isolates of certain serotypes including O1:K69 and O3:K72 possessed both trh and trh and tdh genes. Among these, most of O1:K69 and O3:K72 were urease producer. From these results, we speculate that urease production is closely related to the presence of the trh gene or/and lesser production of TDH. We also found the new combination of serovar, O3:K25, O4:K37 and O13:K72 in Kanagawa phenomenon-positive strains.
我们分析了467株副溶血性弧菌，比较其tdh/trh基因的携带情况、脲酶产生情况及血清型。携带tdh+/trh-、tdh+/trh+、tdh-/trh+和tdh-/trh-的菌株脲酶产生阳性率分别为2.1%、100%、65.7%、100%。O1:K69、O3:K6、O3:K72、O6:K18、O6:K46和O1:KUT血清型的脲酶产生通常呈阳性（除最后一种为91.7%外，其余均为100%）。所有O3:K6分离株均携带trh，而包括O1:K69和O3:K72在内的某些血清型的所有分离株均同时携带trh和tdh基因。其中，大多数O1:K69和O3:K72分离株可产生脲酶。根据这些结果我们推测，脲酶产生与trh基因的存在或/和TDH产生较少密切相关。我们还在神奈川现象阳性菌株中发现了新的血清型组合，即O3:K25、O4:K37和O13:K72。
