Obata H, Kai A, Sekiguchi K, Matsushita S, Yamada S, Itoh T, Ohta K, Kudoh Y
Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health.
Kansenshogaku Zasshi. 1996 Aug;70(8):815-20. doi: 10.11150/kansenshogakuzasshi1970.70.815.
A total of 478 Vibrio parahaemolyticus strains isolated from overseas travellers' diarrhea during the last 7 years of 1989 to 1995 were examined for the production of Kanagawa hemolysin by reversed passive latex agglutination (RPLA) test. Three hundred-seventy (77.4%) out of 478 strains were positive for Kanagawa hemolysin, whereas 108 strains were weakly positive or negative. For those Kanagawa hemolysin-weakly positive or negative strains, the tdh and trh genes associated with the production of TDH (thermostable direct hemolysin) and TRH (TDH-related hemolysin), respectively, were studied by the polymerase chain reaction method. The trh gene was detected in 98 (90.7%) out of 108 strains. In 35 strains belonging to 13 serotypes such as O3: K6, O1:K33, O3:K59, the trh gene alone was detected. On the other hand, both trh and tdh genes were detected in 63 strains of 17 serotypes including O1:K69, O3:K72, O6:K46. Among the strains of 4 serotypes including O1:K56, O1:KUT, O3:KUT and O5:KUT, two types of the trh positive alone and the trh and tdh positive were observed. Of interest, all of the 98 trh-positive strains were positive for the urease hydrolysis, whereas all Kanagawa hemolysin-positive strains were not. Furthermore, the strains of serotype O6:K18 (4 strains) were positive for the fermentation of dulcitol, and the strains of serotype O1:K1 (5 strains) were indole negative. These characteristics of the strains were completely different from those of typical V. parahaemolyticus strains.
对1989年至1995年最后7年期间从海外旅行者腹泻中分离出的478株副溶血性弧菌进行了反向被动乳胶凝集试验（RPLA），以检测其是否产生神奈川溶血素。478株菌株中，有370株（77.4%）神奈川溶血素呈阳性，而108株呈弱阳性或阴性。对于那些神奈川溶血素弱阳性或阴性的菌株，采用聚合酶链反应方法研究了分别与TDH（耐热直接溶血素）和TRH（TDH相关溶血素）产生相关的tdh和trh基因。108株菌株中有98株（90.7%）检测到trh基因。在属于O3:K6、O1:K33、O3:K59等13个血清型的35株菌株中，仅检测到trh基因。另一方面，在包括O1:K69、O3:K72、O6:K46在内的17个血清型的63株菌株中，同时检测到trh和tdh基因。在包括O1:K56、O1:KUT、O3:KUT和O5:KUT在内的4个血清型的菌株中，观察到单独trh阳性和trh及tdh阳性两种类型。有趣的是，所有98株trh阳性菌株脲酶水解均呈阳性，而所有神奈川溶血素阳性菌株则不然。此外，O6:K18血清型的菌株（4株）对卫矛醇发酵呈阳性，O1:K1血清型的菌株（5株）吲哚阴性。这些菌株的这些特征与典型的副溶血性弧菌菌株完全不同。
