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用于微量血清学活性多糖和寡糖定量的被动溶血抑制试验。

Passive hemolysis inhibition test for quantitation of serologically active polysaccharides and oligosaccharides in micro amounts.

作者信息

Kontrohr T, Péterffy K K

出版信息

J Immunol Methods. 1976;13(3-4):271-7. doi: 10.1016/0022-1759(76)90074-0.

DOI:10.1016/0022-1759(76)90074-0
PMID:796388
Abstract

An improved quantitative micromethod is described for the assay of antigenically active bacterial lipopolysaccharides and their immunodeterminant oligosaccharides. The method which is capable of measuring antigens in nanogram quantities yields the same sensitivity as the microcomplement fixation without the limitations of this method. The use of the method was demonstrated on Shigella sonnei phase 1 and phase ii lipopolysaccharide antigens and immunodeterminants.

摘要

本文描述了一种改进的定量微量方法,用于测定具有抗原活性的细菌脂多糖及其免疫决定寡糖。该方法能够测量纳克量的抗原,其灵敏度与微量补体结合法相同,但没有该方法的局限性。该方法已在宋内志贺氏菌1相和2相脂多糖抗原及免疫决定簇上得到验证。

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Passive hemolysis inhibition test for quantitation of serologically active polysaccharides and oligosaccharides in micro amounts.用于微量血清学活性多糖和寡糖定量的被动溶血抑制试验。
J Immunol Methods. 1976;13(3-4):271-7. doi: 10.1016/0022-1759(76)90074-0.
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Serological analysis of core oligosaccharides of Shigella flexneri serotype 6 and its R mutants lipopolysaccharides.福氏志贺菌6型及其R突变体脂多糖核心寡糖的血清学分析。
Arch Immunol Ther Exp (Warsz). 1981;29(5):567-72.
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Synthesis of the repeating unit of the O-specific polysaccharide of Shigella sonnei and quantitation of its serologic activity.宋内志贺氏菌O-特异性多糖重复单元的合成及其血清学活性的定量分析。
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Heterogeneity of Shigella sonnei phase I O-antigen.宋内志贺菌I相O抗原的异质性
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Immunochemical characteristics of Shigella sonnei and serotype 6 Shigella flexneri lipopolysaccharides and enterobacterial common antigen.宋内志贺菌、6型福氏志贺菌脂多糖及肠杆菌共同抗原的免疫化学特性
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Comparative studies on the immunologic properties of free endotoxin and lipopolysaccharide from Shigella sonnei.宋内志贺氏菌游离内毒素与脂多糖免疫特性的比较研究
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Characterization and diagnostic application of a lipopolysaccharide core oligosaccharide-protein conjugate.脂多糖核心寡糖-蛋白质缀合物的表征及诊断应用
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Interferon induction in mice by uniform salt forms of Shigella sonnei lipopolysaccharide and its components.宋内志贺氏菌脂多糖及其组分的均匀盐形式在小鼠中诱导干扰素的产生。
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Biological properties of lipid A from Shigella sonnei.宋内志贺氏菌脂多糖A的生物学特性
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