Kontrohr T, Péterffy K K
J Immunol Methods. 1976;13(3-4):271-7. doi: 10.1016/0022-1759(76)90074-0.
An improved quantitative micromethod is described for the assay of antigenically active bacterial lipopolysaccharides and their immunodeterminant oligosaccharides. The method which is capable of measuring antigens in nanogram quantities yields the same sensitivity as the microcomplement fixation without the limitations of this method. The use of the method was demonstrated on Shigella sonnei phase 1 and phase ii lipopolysaccharide antigens and immunodeterminants.
本文描述了一种改进的定量微量方法,用于测定具有抗原活性的细菌脂多糖及其免疫决定寡糖。该方法能够测量纳克量的抗原,其灵敏度与微量补体结合法相同,但没有该方法的局限性。该方法已在宋内志贺氏菌1相和2相脂多糖抗原及免疫决定簇上得到验证。