Fiddy C, Trinci A P
J Gen Microbiol. 1976 Dec;97(2):169-84. doi: 10.1099/00221287-97-2-169.
Mitosis, septation and branching were studied in undifferentiated mycelia and leading hyphae of Aspergillus nidulans, a mould which forms incomplete septa. After spore germination, nuclei divided synchronously until germ-tube hyphae contained 8 or 16 nuclei; mitosis occurred when the volume of cytoplasm per haploid nucleus was about 60 mum3. Intercompartment development was not synchronized, consequently mitosis in the mycelium as a whole eventually became asynchronous. During the stage of asynchronous compartment development, the nuclei, septa, branches and total length of undifferentiated mycelia all increased exponentially at approximately the same specific rate. Septa were formed in hyphae in groups of up to nine; the mean time required for the formation of a group of septa was about 9 min. The mean interval between successive cycles of septation in a hypha was approximately the same as the organism's doubling time. There was a highly significant correlation coefficient between septation and branch initiation and most intercalary compartments initially formed a single branch. The volume of cytoplasm per nucleus in a diploid strain was approximately double the value observed for a haploid strain. However, the length of the hyphal growth unit was not affected by ploidy. The study suggests that a duplication cycle can be recognized during mycelial growth which is analogous to the cell cycle observed in unicellular micro-organisms.
对构巢曲霉的未分化菌丝体和前端菌丝中的有丝分裂、隔膜形成和分支进行了研究，构巢曲霉是一种形成不完全隔膜的霉菌。孢子萌发后，细胞核同步分裂，直到芽管菌丝含有8个或16个细胞核；当每个单倍体细胞核的细胞质体积约为60μm³时发生有丝分裂。隔室间的发育不同步，因此整个菌丝体中的有丝分裂最终变得不同步。在不同步的隔室发育阶段，未分化菌丝体的细胞核、隔膜、分支和总长度均以大致相同的特定速率呈指数增长。菌丝中隔膜成组形成，每组最多9个；形成一组隔膜所需的平均时间约为9分钟。菌丝中连续隔膜形成周期之间的平均间隔与生物体的倍增时间大致相同。隔膜形成与分支起始之间存在高度显著的相关系数，并且大多数居间隔室最初形成单个分支。二倍体菌株中每个细胞核的细胞质体积约为单倍体菌株观察值的两倍。然而，菌丝生长单位的长度不受倍性的影响。该研究表明，在菌丝体生长过程中可以识别出一个复制周期，它类似于在单细胞微生物中观察到的细胞周期。
