Cohen J I, Seidel K E
Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
J Gen Virol. 1994 Nov;75 ( Pt 11):3087-93. doi: 10.1099/0022-1317-75-11-3087.
Varicella-zoster virus (VZV) encodes at least five glycoproteins, gpI to gpV. VZV gpV, M(r) 100K to 110K, is the product of VZV open reading frame (ORF) 14. VZV gpV is homologous to herpes simplex virus gC and pseudorabies virus gIII. To determine whether gpV is required for viral replication, we inserted a stop codon after the fifteenth codon of the ORF14 gene in a cosmid containing the gene. Transfection of human melanoma cells with the cosmid containing the mutant ORF14 gene and three other cosmids resulted in the production of infectious VZV. Immunoprecipitation indicated that the mutant virus did not express gpV. VZV that did not express gpV grew at the same rate as parental virus and was inhibited by heparin to a similar extent. The pattern of inhibition by heparin of the gpV mutant was similar to that reported for a herpes simplex virus mutant that does not contain gC, but different from that described for a pseudorabies virus mutant devoid of gIII. These results indicate that VZV gpV is not required for viral replication in vitro.
水痘带状疱疹病毒（VZV）编码至少五种糖蛋白，即糖蛋白I至糖蛋白V。VZV糖蛋白V，分子量为100K至110K，是VZV开放阅读框（ORF）14的产物。VZV糖蛋白V与单纯疱疹病毒gC和伪狂犬病病毒gIII同源。为了确定糖蛋白V是否是病毒复制所必需的，我们在含有该基因的黏粒中，于ORF14基因的第15个密码子之后插入了一个终止密码子。用含有突变型ORF14基因的黏粒和其他三种黏粒转染人黑色素瘤细胞，产生了有传染性的VZV。免疫沉淀表明突变病毒不表达糖蛋白V。不表达糖蛋白V的VZV生长速度与亲本病毒相同，并且被肝素抑制的程度相似。肝素对糖蛋白V突变体的抑制模式与报道的不含gC的单纯疱疹病毒突变体相似，但与缺乏gIII的伪狂犬病病毒突变体的情况不同。这些结果表明，VZV糖蛋白V在体外病毒复制过程中并非必需。
