Weclewicz K, Kristensson K, Svensson L
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
J Gen Virol. 1994 Nov;75 ( Pt 11):3267-71. doi: 10.1099/0022-1317-75-11-3267.
The effect of rotavirus infection on cytoskeletal organization was examined in cultured African green monkey kidney (CV-1) cells. Rhesus rotavirus caused significant and selective changes in the organization of the vimentin filament network without having any effect on microtubules or actin. Double-immunofluorescence studies showed that at 6 h post-infection, and in the absence of cytopathic effect, the normal arrays of vimentin fibres radiating from multiple sites around the nucleus were lost. Vimentin fibres became irregularly distributed in the cytoplasm and were totally disrupted in the later stages of infection. Vimentin reorganization occurred independent of extracellular Ca2+ levels.
在培养的非洲绿猴肾（CV-1）细胞中检测了轮状病毒感染对细胞骨架组织的影响。恒河猴轮状病毒导致波形蛋白丝网络的组织发生显著且选择性的变化，而对微管或肌动蛋白没有任何影响。双重免疫荧光研究表明，在感染后6小时，且在没有细胞病变效应的情况下，从细胞核周围多个位点放射状排列的波形蛋白纤维正常阵列消失。波形蛋白纤维在细胞质中分布不规则，并在感染后期完全被破坏。波形蛋白的重组独立于细胞外Ca2+水平发生。
