Manley G, Wong E, Dalmau J, Elkon K, Posner J, Furneaux H
Laboratory of Molecular Neuro-Oncology, Memorial Sloan-Kettering Cancer Center, N.Y., N.Y.
J Neurooncol. 1994;19(2):105-12. doi: 10.1007/BF01306451.
Screening a small cell lung cancer cDNA library with serum from a patient with antibody-associated paraneoplastic sensory neuronopathy (Anti-Hu syndrome) resulted in the isolation of a cDNA clone encoding the Ro-52 kD antigen. The Ro-52 kD antigen is one of the major antigens recognized by the sera of patients with Sjögren's syndrome and systemic lupus erythematosus. Further investigation revealed that the sera of only a small percentage (4%) of patients with the Hu syndrome reacted with Ro-52 kD antigen. The cross-reactivity may result from a domain of high homology between Ro-52 kD and HuD antigen. These results emphasize the necessity of using recombinant antigens whenever possible when assaying for assaying for specific antibodies.
用一名患有抗体相关性副肿瘤性感觉神经元病（抗Hu综合征）患者的血清筛选小细胞肺癌cDNA文库，分离出了一个编码Ro - 52 kD抗原的cDNA克隆。Ro - 52 kD抗原是干燥综合征和系统性红斑狼疮患者血清识别的主要抗原之一。进一步研究发现，只有一小部分（4%）Hu综合征患者的血清与Ro - 52 kD抗原发生反应。这种交叉反应可能是由于Ro - 52 kD和HuD抗原之间存在高度同源的结构域。这些结果强调了在检测特异性抗体时尽可能使用重组抗原的必要性。
