Polewski K, Zinger D, Trunk J, Monteleone D C, Sutherland J C
Biology Department, Brookhaven National Laboratory, Upton, NY 11973.
J Photochem Photobiol B. 1994 Aug;24(3):169-77. doi: 10.1016/1011-1344(94)07018-0.
We have prepared argon and nitrogen matrices containing guanine and 7-methylguanine, and measured their absorption, fluorescence excitation and fluorescence emission spectra. The fluorescence excitation spectrum of guanine shows four well-resolved bands in the range from 170 to 290 nm; excitation at the wavelengths of each of these bands results in a fluorescence emission with maximum intensity near 350 nm and a single-exponential decay with a lifetime of about 10 ns. There are significant differences between the fluorescent excitation and emission spectra of guanine and of 7-methylguanine, suggesting that the fluorescence observed from the guanine sample does not arise from a minority tautomer.
我们制备了含有鸟嘌呤和7-甲基鸟嘌呤的氩气和氮气基质，并测量了它们的吸收光谱、荧光激发光谱和荧光发射光谱。鸟嘌呤的荧光激发光谱在170至290nm范围内显示出四个分辨率良好的谱带；在这些谱带各自的波长处激发会导致在350nm附近具有最大强度的荧光发射以及约10ns寿命的单指数衰减。鸟嘌呤和7-甲基鸟嘌呤的荧光激发光谱和发射光谱之间存在显著差异，这表明从鸟嘌呤样品中观察到的荧光并非来自少数互变异构体。
