Gryczynski I, Cherek H, Lakowicz J R
University of Maryland, School of Medicine, Department of Biological Chemistry, Baltimore 21201.
Biophys Chem. 1988 Jul 15;30(3):271-7. doi: 10.1016/0301-4622(88)85022-1.
We measured the frequency response of the polarized emission of Yt-base in propylene glycol at 10 degrees C. Data were obtained for excitation wavelengths of 290, 312 and 346 nm, for which the fundamental anisotropies are 0.05, 0.19 and 0.32, respectively. Additionally, data were obtained using CCl4, to decrease the mean decay time from 9.1 to 4.2 ns. These nine sets of data were analyzed globally to recover the anisotropy decay law. Three correlation times were needed to fit the data, 0.8, 3.0 and 5.6 ns, a range of only 7-fold. We believe this is the first reported detection of three correlation times for a rigid molecule.
我们测量了10摄氏度下丙二醇中钇基偏振发射的频率响应。获得了激发波长为290、312和346纳米的数据，其基本各向异性分别为0.05、0.19和0.32。此外，使用四氯化碳获得了数据，以将平均衰减时间从9.1纳秒降至4.2纳秒。对这九组数据进行了整体分析，以恢复各向异性衰减规律。需要三个相关时间来拟合数据，分别为0.8、3.0和5.6纳秒，范围仅为7倍。我们认为这是首次报道对刚性分子检测到三个相关时间。
