Chaet M S, Arya G, Ziegler M M, Warner B W
Department of Surgery, Children's Hospital Medical Center, University of Cincinnati College of Medicine, OH 45229-3039.
J Pediatr Surg. 1994 Aug;29(8):1035-8; discussion 1038-9. doi: 10.1016/0022-3468(94)90274-7.
Epidermal growth factor (EGF) has documented trophic effects on several tissues, including small bowel (SB) mucosa. The purpose of this study was to determine the effect of EGF on intestinal adaptation after massive resection. Young Sprague-Dawley rats (weight, 154 +/- 8.6 g) underwent a 75% proximal SB resection, with primary reanastomosis, and were continuously infused with equivalent volumes of either saline or human recombinant EGF (6.25 micrograms/kg/h) via a subcutaneous osmotic pump. In other animals, the SB was transected and reanastomosed without resection and either saline or EGF was infused. The animals were allowed standard rat chow and water ad libitum and were killed after 28 days. SB was harvested and subjected to histological examination. Intestinal mucosa was analyzed for total DNA and protein contents. After massive SB resection, subcutaneous administration of EGF resulted in significantly increased animal weight (294.5 +/- 16 v 242.8 +/- 14g; P < .05), SB weight (52.8 +/- 7.0 v 37.2 +/- 6.0 cm; P < .05), SB length (14.0 +/- 1.3 v 7.4 +/- 1.6 g; P < .05), and mucosal thickness (1.04 +/- 0.2 v 0.68 +/- 0.2 mm; P < .05). DNA and protein analyses suggest that EGF may augment the mucosal hyperplasia response to massive SB resection. Administration of EGF after massive SB loss may be of nutritional benefit to the host through its enhancement of the normal postresection intestinal response.
表皮生长因子（EGF）已被证明对包括小肠（SB）黏膜在内的多种组织具有营养作用。本研究的目的是确定EGF对大面积切除术后肠道适应性的影响。将年轻的Sprague-Dawley大鼠（体重154±8.6 g）进行75%近端小肠切除并一期吻合，通过皮下渗透泵持续输注等量的生理盐水或重组人EGF（6.25微克/千克/小时）。在其他动物中，小肠进行横断和吻合但不切除，并输注生理盐水或EGF。动物自由摄取标准大鼠饲料和水，28天后处死。采集小肠进行组织学检查。分析肠黏膜的总DNA和蛋白质含量。大面积小肠切除术后，皮下给予EGF可使动物体重（294.5±1б比242.8±14 g；P<0.05）、小肠重量（52.8±7.0比37.2±6.0 cm；P<0.05）、小肠长度（14.0±1.3比7.4±1.6 g；P<0.05）和黏膜厚度（1.04±0.2比0.68±0.2 mm；P<0.05）显著增加。DNA和蛋白质分析表明，EGF可能增强对大面积小肠切除的黏膜增生反应。大面积小肠切除后给予EGF可能通过增强正常的切除后肠道反应对宿主具有营养益处。
