Gandelman M S, Baldwin R M, Zoghbi S S, Zea-Ponce Y, Innis R B
Department of Psychiatry, Yale University School of Medicine, CT.
J Pharm Sci. 1994 Jul;83(7):1014-9. doi: 10.1002/jps.2600830718.
An ultrafiltration system was evaluated for the free-fraction measurement of SPECT radiotracers (beta-CIT, IBF, and iomazenil) used in functional brain imaging. The effect of temperature, storage, centrifugal force, tracer concentration, and percentage filtered demonstrated a relative error of < 9%. As a result of the minimal temperature effect, 25 degrees C was employed for all measurements. A comparison of the ultrafiltration system with equilibrium dialysis revealed < 5% difference for beta-CIT and iomazenil, but 16% for IBF. Additionally, the time and ease of operation considerably favored the ultrafiltration system. The precision quantitated by repetition was < 6% for between-run and within-run variability. In conclusion, ultrafiltration provided rapid results, demonstrated minor analytical errors, revealed generally good correlation with equilibrium dialysis, and allowed excellent precision.
对一种超滤系统进行了评估，用于测量功能性脑成像中使用的SPECT放射性示踪剂（β-CIT、IBF和碘美普尔）的游离分数。温度、储存、离心力、示踪剂浓度和过滤百分比的影响显示相对误差<9%。由于温度影响极小，所有测量均采用25摄氏度。超滤系统与平衡透析的比较显示，β-CIT和碘美普尔的差异<5%，但IBF的差异为16%。此外，超滤系统在时间和操作简便性方面具有很大优势。通过重复定量的精密度，批间和批内变异均<6%。总之，超滤提供了快速的结果，显示出较小的分析误差，与平衡透析总体相关性良好，并具有出色的精密度。
