Barnakov A N
Biochemistry/Biophysics Department, Washington State University, Pullman 99164-4660.
J Microsc. 1994 Aug;175(Pt 2):171-4. doi: 10.1111/j.1365-2818.1994.tb03481.x.
A simple procedure for screening by electron microscopic observations of conditions for the reconstitution of membrane proteins into lipid bilayers is described. This procedure consists of a 5-10 s treatment of electron microscopic grids, to which the sample has already been applied, with 1% phosphotungstic acid before proceeding with final staining in uranyl acetate. The method substantially enhances the adherence of lipid membranes and membrane protein particles to hydrophobic collodion/carbon grids.
描述了一种通过电子显微镜观察将膜蛋白重组到脂质双层中的条件进行筛选的简单程序。该程序包括在进行醋酸铀最终染色之前，用1%磷钨酸对已经施加了样品的电子显微镜网格进行5-10秒的处理。该方法显著增强了脂质膜和膜蛋白颗粒对疏水火棉胶/碳网格的附着力。
