Jenö S, Tibor S, György L, György K, Lajos L
Morphol Igazsagugyi Orv Sz. 1976 Oct;16(4):270-3.
Authors describe their own method for the investigation of needle-biopsy of kidney using light-, and electron microscopy and immunofluorescent technique as well. Small pieces are cut from both end of the needle-biopsy-cylinder and are embedded for electron microscopy. From the larger central part of it cryostat sections are made (at -20 degrees C) for the immunofluorescent study. Afterwards the piece of tissue remained can be fixed in formaldehyde and embedded in paraffin for light microscopic investigation. All three methods can be carried out even when having a 6 mm long needle-biopsy specimen.
作者描述了他们自己使用光学显微镜、电子显微镜以及免疫荧光技术对肾穿刺活检进行研究的方法。从肾穿刺活检圆柱体的两端切取小块组织用于电子显微镜包埋。从其较大的中央部分制作低温切片(在-20摄氏度)用于免疫荧光研究。之后,剩余的组织块可以用甲醛固定并石蜡包埋用于光学显微镜检查。即使只有6毫米长的肾穿刺活检标本,这三种方法都可以实施。