Sato C, Furuya K
Hokkaido Institute of Public Health, Sapporo, Japan.
Jpn J Med Sci Biol. 1994 Feb;47(1):65-71. doi: 10.7883/yoken1952.47.65.
A diagnostic antigen, C-antigen, was evaluated in serodiagnosis of alveolar hydatid disease by Western blotting. The C-antigen migrated diffusely to the region of 30-35 kDa in 8% polyacrylamide-SDS gel electrophoresis. We isolated this antigen from a crude metacestode extract by a simple method of phenol/chloroform extraction. The isolated antigen was resistant to boiling, proteolysis and acid hydrolysis, but destroyed with sodium periodate. Such lectins as peanut agglutinin (PNA), Ricinus communis lectin (RCA120) and wheatgerm agglutinin (WGA) were capable of binding with the isolated antigen; no binding to concanavalin A (Con A), Lens culinaris agglutinin (LCA) or Phaseolus vulgaris (PHA-E4) was demonstrated. C-antigen is associated with a polysaccharide containing galactose and N-acetyl glucosamine residues.
通过蛋白质印迹法对一种诊断抗原C抗原在肺泡包虫病血清诊断中的应用进行了评估。在8%聚丙烯酰胺 - SDS凝胶电泳中，C抗原呈弥散状迁移至30 - 35 kDa区域。我们通过一种简单的苯酚/氯仿提取法从粗制的原头蚴提取物中分离出了这种抗原。分离出的抗原对煮沸、蛋白水解和酸水解具有抗性，但可被高碘酸钠破坏。诸如花生凝集素（PNA）、蓖麻凝集素（RCA120）和麦胚凝集素（WGA）等凝集素能够与分离出的抗原结合；未显示与刀豆球蛋白A（Con A）、扁豆凝集素（LCA）或菜豆凝集素（PHA - E4）有结合。C抗原与一种含有半乳糖和N - 乙酰葡糖胺残基的多糖相关。
