Enomoto Y, Shiiki H, Nishino T, Dohi K
First Department of Internal Medicine, Nara Medical University, Japan.
Nihon Jinzo Gakkai Shi. 1994 Sep;36(9):1021-7.
We investigated the intraglomerular distribution of thrombomodulin (TM) antigen in patients with various renal diseases. The subjects enrolled in this study were 28 patients with IgA nephropathy, 26 with collagen diseases, 10 with toxemia and 4 with DIC. Normal renal cortex used as a control was obtained from the normal pole of kidneys with a tumor of the opposite pole. Intraglomerular distribution of TM antigen was detected by an immunohistochemical method using a polyclonal antibody against human TM. The following results were obtained: 1) The staining intensity of TM on endothelial cells of glomerular tufts was higher in IgA nephropathy and collagen diseases than in the controls, but was the same in toxemia and DIC as in the controls. 2) The staining intensity of TM decreased with the progression of the glomerular lesion in IgA nephropathy and lupus nephritis. These findings suggest that the intraglomerular distribution of TM may be involved in the progression of glomerular lesions and in the acceleration of intraglomerular blood coagulation in various renal diseases.
我们研究了各种肾脏疾病患者肾小球内血栓调节蛋白（TM）抗原的分布情况。本研究纳入的受试者包括28例IgA肾病患者、26例胶原病患者、10例毒血症患者和4例弥散性血管内凝血（DIC）患者。用作对照的正常肾皮质取自对侧肾极有肿瘤的肾脏的正常极。使用抗人TM的多克隆抗体通过免疫组织化学方法检测TM抗原在肾小球内的分布。获得了以下结果：1）IgA肾病和胶原病患者肾小球毛细血管襻内皮细胞上TM的染色强度高于对照组，但毒血症和DIC患者与对照组相同。2）在IgA肾病和狼疮性肾炎中，TM的染色强度随肾小球病变的进展而降低。这些发现表明，TM在肾小球内的分布可能与各种肾脏疾病中肾小球病变的进展以及肾小球内血液凝固的加速有关。
